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. 2018 Aug 27;8:12912. doi: 10.1038/s41598-018-30918-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The transposable element insertion is only observed in the analysed double flower roses. PCR to detect the TE were performed on genomic DNA from different rose cultivars exhibiting simple (b) or double flowers deriving from R. chinensis (a). The lower band (419 bp) corresponds to the combination of primers that amplifies the wild type allele, while the two higher ones (770 bp and 754 bp) correspond to amplification of the left and right borders of the transposable element insertion, respectively.