Table 2.

Gradient Program for Positive and negative Total Lipid Runs.

Time	% Solvent A	% Solvent B	Flow Rate (nl/min)
00:00	70	30	250
01:00	50	50	250
40:00	2	98	250
50:00	2	98	250
50:01	70	30	250
65:01	70	30	250