Fig. 5.

Cyst permeabilization by V. cholerae depends on its lecithinase. a Representative images and b quantification of lysed or non-lysed cysts that were infected by WT, lecithinase-minus (Δlec), lecithinase-minus but complemented (Δlec::lec), and lec-merodiploid V. cholerae strains (all GFP positive). The permeabilization phenotype was visualized through the infiltration of dextran from the surrounding medium in the white arrow-marked cysts, which did not occur for the Δlec strain. Scale bar: 10 µm. The graph in b represents average values from three independent biological replicates (±s.d.). Statistics are based on a one-way ANOVA with ***p ≤ 0.001; **p ≤ 0.01; ns, p > 0.05