Table 1.

VL-17A cells were treated for 48 h with control- or ethanol-containing media supplemented with BSA, oleate, palmitate or C2 ceramide.

		BSA	Oleate		Palmitate		C2 Ceramide
		AVG ± SEM	AVG ± SEM	p	AVG ± SEM	p	AVG ± SEM	p
Control	PPARα	1.00 ± 0.22	1.02 ± 0.10	1.00	0.82 ± 0.12	0.97	0.99 ± 0.18	0.98
	CPT1	1.00 ± 0.13	1.82 ± 0.07	0.57	0.86 ± 0.10	0.65	0.92 ± 0.09	0.77
	MCD1	0.99 ± 0.00	1.05 ± 0.11	0.99	0.69 ± 0.11	0.78	1.31 ± 0.39	0.95
	SREBP-1C	1.00 ± 0.09	1.53 ± 0.35	0.89	0.97 ± 0.13	0.99	1.18 ± 0.19	0.94
	FAS	1.00 ± 0.09	1.27 ± 0.10	0.23	1.31 ± 0.34	0.85	1.50 ± 0.14	0.10
	ACC1	1.00 ± 0.05	0.92 ± 0.12	0.96	0.69 ± 0.11	0.78	1.31 ± 0.19	0.33
Ethanol	PPARα	1.04 ± 0.07	1.02 ± 0.19	1.00	0.87 ± 0.08	0.97	2.86 ± 0.26	0.01
	CPT1	1.15 ± 0.12	2.14 ± 0.79	0.48	1.20 ± 0.09	0.78	2.03 ± 0.22	0.02
	MCD1	0.96 ± 0.08	1.01 ± 0.08	0.99	1.32 ± 0.43	0.78	1.28 ± 0.14	0.95
	SREBP-1C	1.24 ± 0.11	0.77 ± 0.12	0.89	1.00 ± 0.10	0.78	1.16 ± 0.20	0.94
	FAS	1.04 ± 0.21	1.09 ± 0.08	0.24	0.61 ± 0.20	0.80	0.99 ± 0.09	0.94
	ACC1	0.90 ± 0.14	1.02 ± 0.06	0.96	1.32 ± 0.43	0.78	1.36 ± 0.07	0.25

Isolated mRNA was reverse transcribed and transcript levels were determined using real-time RT-PCR and normalized to 18S RNA levels. Data presented as mean +/−SEM (N = 3–5). p values were calculated relative to BSA control by ANOVA.