Table 1.
BSA | Oleate | Palmitate | C2 Ceramide | |||||
---|---|---|---|---|---|---|---|---|
AVG ± SEM | AVG ± SEM | p | AVG ± SEM | p | AVG ± SEM | p | ||
Control | PPARα | 1.00 ± 0.22 | 1.02 ± 0.10 | 1.00 | 0.82 ± 0.12 | 0.97 | 0.99 ± 0.18 | 0.98 |
CPT1 | 1.00 ± 0.13 | 1.82 ± 0.07 | 0.57 | 0.86 ± 0.10 | 0.65 | 0.92 ± 0.09 | 0.77 | |
MCD1 | 0.99 ± 0.00 | 1.05 ± 0.11 | 0.99 | 0.69 ± 0.11 | 0.78 | 1.31 ± 0.39 | 0.95 | |
SREBP-1C | 1.00 ± 0.09 | 1.53 ± 0.35 | 0.89 | 0.97 ± 0.13 | 0.99 | 1.18 ± 0.19 | 0.94 | |
FAS | 1.00 ± 0.09 | 1.27 ± 0.10 | 0.23 | 1.31 ± 0.34 | 0.85 | 1.50 ± 0.14 | 0.10 | |
ACC1 | 1.00 ± 0.05 | 0.92 ± 0.12 | 0.96 | 0.69 ± 0.11 | 0.78 | 1.31 ± 0.19 | 0.33 | |
Ethanol | PPARα | 1.04 ± 0.07 | 1.02 ± 0.19 | 1.00 | 0.87 ± 0.08 | 0.97 | 2.86 ± 0.26 | 0.01 |
CPT1 | 1.15 ± 0.12 | 2.14 ± 0.79 | 0.48 | 1.20 ± 0.09 | 0.78 | 2.03 ± 0.22 | 0.02 | |
MCD1 | 0.96 ± 0.08 | 1.01 ± 0.08 | 0.99 | 1.32 ± 0.43 | 0.78 | 1.28 ± 0.14 | 0.95 | |
SREBP-1C | 1.24 ± 0.11 | 0.77 ± 0.12 | 0.89 | 1.00 ± 0.10 | 0.78 | 1.16 ± 0.20 | 0.94 | |
FAS | 1.04 ± 0.21 | 1.09 ± 0.08 | 0.24 | 0.61 ± 0.20 | 0.80 | 0.99 ± 0.09 | 0.94 | |
ACC1 | 0.90 ± 0.14 | 1.02 ± 0.06 | 0.96 | 1.32 ± 0.43 | 0.78 | 1.36 ± 0.07 | 0.25 |
Isolated mRNA was reverse transcribed and transcript levels were determined using real-time RT-PCR and normalized to 18S RNA levels. Data presented as mean +/−SEM (N = 3–5). p values were calculated relative to BSA control by ANOVA.