FIGURE 3.

SOX2 inhibition reduced tumorigenesis and metastasis in xenograft model. (A) Tumor xenografts in mice. MDA-MB-231 cells infected with sh-SOX2 or sh-control were subcutaneously injected into nude mice (6 in each group). 28 days later, the mice were sacrificed and dissected, and the tumors were weighed. (B) Tumor metastasis in mouse xenograft models. MDA-MB-231 cells infected with sh-SOX2 or sh-control lentivirus were injected into the tail vein of nude mice (5 in each group). 28 days later, the mice were sacrificed and micrometastases in the lung per HE-stained section from individual mice were calculated. (C) Expression levels of SOX2 in the mouse xenograft model. The expression level of SOX2 in the sh-SOX2 group was significantly lower than that in the sh-CTR group.