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. 2018 Aug 21;9:1970. doi: 10.3389/fmicb.2018.01970

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Copyright © 2018 Hofmann, Otto, Berges, Biedendieck, Michel, Becher, Jahn and Neumann-Schaal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth curve of C. difficile 630Δerm. Shown is the growth curve of C. difficile 630Δerm in casamino acids medium of four biological replicates, lag phase corrected, and the sampling time points (^∗) in the exponential phase (exp), the transient phase (trans), and three times in the stationary phase (stat1-3). The curve was fitted according to the biphasic Hill Equation using OriginPro, 2016 software (OriginLab Corporation, MA, USA); reduction of the optical density after time point stat1 was induced by aggregation of the cells in the culture medium.