Figure 3.

Flutamide inhibition of androgen-induced maturation and p42 phosphorylation. Oocytes were incubated in MBSH in the presence or absence of flutamide with deoxycorticosterone (DOC) (A), progesterone (B), testosterone (C), or AD (D). Similar experiments were performed 3, 5, 5, and 3 times, respectively, with nearly identical results. (E) Ooocytes were treated as above except incubated with the indicated steroid at 50 nM for 4 h. Blots were probed with antiserum against phosphorylated p42 protein (Upper) followed by antiserum against total p42 protein (Lower). Twenty oocytes were used for each sample, and similar experiments were performed three times with equivalent results.