Skip to main content
. 2018 Aug 21;9:1895. doi: 10.3389/fmicb.2018.01895

FIGURE 1.

FIGURE 1

Characterization of lung derived dendritic cell cultures. (A) Phase contrast microscopy of CD11c positive cells isolated from lungs of Balb/c mice and cultured during 1, 7, and 15 days in the presence of 20 ng/mL GM-CSF. Media was replenished every 48 h. (B) Cell density expressed as total cell number/T75 flask after culturing isolated CD11c positive cells during 0, 10, 20, 30, 40, and 50 days in the presence of GM-CSF, p > 0.05. (C) Cell viability of CD11c cultures expressed as percentage of viable cells after culturing 0, 7, 10, 13, 20, and 28 days in the presence of GM-CSF, p > 0.05. (D) Phenotype of CD11c positive cells after cultured as above for 15 days were stained with fluorescence labeled mAb CD11b/CD11c and analyzed by flow cytometry. The gating strategy used selected cells based on forward and size scatter area (FSC-A/SSC-A) followed by analysis of intensity of fluorescence for CD11c and CD11b expression. The quadrants were set using unstained cells (left dot-plot). Cells stained for CD11c and CD11b are shown in the right dot-plot. Numbers in each quadrant represent the percentage of positive cells. Representative data from more than three experiments in similar conditions.