Table 2.
List of primer sequences of trafficking regulators and lysosomal enzymes used for qRT-PCR.
| Gene | Common name | Forward primer | Reverse primer |
|---|---|---|---|
| Rab1 | Rab1 | 5′-CCTTCAATAACGTTAAACAGT-3′ | 5′-TAGTCTACTACTTTCTTTGTGG-3′ |
| Rab5a | Rab5a | 5′-GTACTACCGAGGAGCACAAG-3′ | 5′-AAGCTGTTGTCATCTGCATAG-3′ |
| Rab5b | Rab5b | 5′-GACTAGCAGAAGTACAGCCAG- 3′ | 5′-CAATGGTGCTTTCCTGGTATTC-3′ |
| Rab7 | Rab7 | 5′-CCTCTAGGAAGAAAGTGTTGC-3′ | 5′-TTCTTGACCGGCTGTGTCCCA- 3′ |
| Rab9 | Rab9 | 5′-GCCCATGCAGATTTGGGACAC-3′ | 5′-GCCGGCTTGGGCTTCTTCTGTA-3′ |
| Rab10 | Rab10 | 5′-GCCGAATGTTACTAGGGAACAAG-3′ | 5′-GCCGCCTCCTCCACTGCTGATA-3′ |
| Rab11 | Rab11 | 5′-GAGCAGTAGG TGCCTTATTGG-3′ | 5′-GAACTGCCCTGAGATGACGTA-3′ |
| Rab14 | Rab14 | 5′-GCCGGAGCTACTATAGAGGAGCT-3′ | 5′-GCCGTTCTGATAGATTTTCTTGG-3′ |
| Rab20 | Rab20 | 5′-CTGCTGCAGCGCTACATGGAGCG- 3′ | 5′-CTCCGCGGCAGTACAGGGAGC-3′ |
| Rab22a | Rab22a | 5′-GCCGACAAGAACGATTTCGTGCA-3′ | 5′-GCCGACTTCTCTGACATCAGTA-3′ |
| Rab24 | Rab24 | 5′-GCGCGGGTGAGCACCGCAGGGC-3′ | 5′-GCCTCAGACCCCAACCCCAAG-3′ |
| Rab31 | Rab31 | 5′-GCCCAGAAAACATTGTGATGGCG-3′ | 5′-GGCATTCTTCGCGCTGGTCTCC-3′ |
| Rab32 | Rab32 | 5′-GCCGAGTATACTATAAGGAAGCTC-3′ | 5′-GCCCTGGGAAGGACTCTGGCTG-3′ |
| Rab34 | Rab34 | 5′-GCAAAGTGACCCCGTGTGGCGGG−3′ | 5′-GGGCGTCCCGAAGACCACTCGG-3′ |
| Eea1 | Early endosome antigen 1 | 5′-GCCCAATGAAGAGTCAGCAAGTC-3′ | 5′-GCCCACCTTGAGATGCTGGCGC-3′ |
| Rilp | Rab-interacting lysosomal protein | 5′-CAGGAACAGCTACAGCGCCTCCT-3′ | 5′-CTGAGGTTGCCGCATCAGGTTC-3′ |
| Lamp1 | Lysosomal membrane glycoprotein 1 | 5′-GGCCGCTGCTCCTGCTGCTGCTG- 3′ | 5′-ATATCCTCTTCCAAAAGTAATTG- 3′ |
| Lamp2 | Lysosomal membrane glycoprotein 2 | 5′-AGGGTACTTGCCTTTATGCAGAAT-3′ | 5′-GTGTCGCCTTGTCAGGTACTGC−3′ |
| Lyz1 | Lysozyme 1 | 5′-CTCTCCTGACTCTGGGACTCCTCC-3′ | 5′-CTGAGCTAAACACACCCAGTCAGC-3′ |
| Lyz2 | Lysozyme 2 | 5′-GGCCAAGGTCTACAATCGTTGTG−3′ | 5′-GCAGAGCACTGCAATTGATCCCA−3′ |
| HexA | Hexosaminidase A | 5′-GCCGGCTGCAGGCTCTGGGTTTC- 3′ | 5′-GCGCGGCCGAACTGACATGGTAC- 3′ |
| HexB | Hexosaminidase B | 5′-CCCGGGCTGCTGCTGCTGCAGGC- 3′ | 5′- GTGGAATTGGGACTGTGGTCGATG- 3′ |
| Hexdc | Hexosaminidase D | 5′-CCACGCCATTTAAGATGAGATTAG-3′ | 5′-GGCCCTCAGCAGCCTCAGGTGGCC-3′ |
| Gla | Galactosidase, α | 5′-GGCCATGAAGCTTTTGAGCAGAG- 3′ | 5′- AGTCAAGGTTGCACATGAAACGTT- 3′ |
| Glb1 | Galactosidase, β1 | 5′-GGAGGTGCAGCGGCTGGCCAGAGC-3′ | 5′-GGTGACATTATAGATGCCGTGCGC-3′ |
| Glb1l | Galactosidase, β1 like | 5′-GTGACGGGTGGGAAAGCCCTCACC-3′ | 5′-CTGTCATGTTCCCGATCCACAACG-3′ |
| Lpl | Lipoprotein lipase | 5′- CAGACATCGAAAGCAAATTTGCCC-3′ | 5′- GTCCATCCATGGATCACCACGAAG-3′ |
| CtsA | Cathepsin A | 5′-GCCCTCCCCGGCCTGGCCAAGCAG-3′ | 5′-GCCGGCTGGATCAGAAAGGGGCCG-3′ |
| CtsB | Cathepsin B | 5′-GCCGTGGTGGTCCTTGATCCTTCTT-3′ | 5′-GCCCCTCACCGAACGCAACCCTTC-3′ |
| CtsC | Cathepsin C | 5′-GCCGCCACACAGCTATCAGTTACTG-3′ | 5′-GCCCCTGGAGACCTCCAAGATGTGC-3′ |
| CtsD | Cathepsin D | 5′-CGTCTTGCTGCTCATTCTCGGCCTC- 3′ | 5′-CACTGGCTCCGTGGTCTTAGGCGAT- 3′ |
| CtsE | Cathepsin E | 5′-GGAGCAGAGTGAGAGAGAAGCTAC-3′ | 5′- GGGCCCGTAGTTTCTTCCGAAGGG-3′ |
| CtsF | Cathepsin F | 5′-GCC GCA GGC TCC GCC TCG-3′ | 5′-GCC GCT CCT AGC ACG GCC-3′ |
| CtsG | Cathepsin G | 5′- CCTGTGCACACCTGTATCTACATAA-3 | 5′- CTGTGTACCGAGTCACCGTACACGC-3′ |
| CtsH | Cathepsin H | 5′- CTGAGAACCCTTCTTCCCAAGAGC−3′ | 5′- AGCAGCCAGGCCCCAGCGCACAGC−3′ |
| CtsK | Cathepsin K | 5′- GGATGAAATCTCTCGGCGTTTAAT-3′ | 5′- GTCTCCCAAGTGGTTCATGGCCAG-3′ |
| CtsL | Cathepsin L | 5′-GCCCCTTTTGGCTGTCCTCTGCTT-3′ | 5′-GCCCTCCATGGAAAAGCCGTGC-3′ |
| CtsO | Cathepsin O | 5′-GCCCGCAGTTGGTGAACCTCTTGCT-3′ | 5′-GCCGTCCTTCTGCTGGGTATCTGGG-3′ |
| CtsS | Cathepsin S | 5′-GCCGACTACCATTGGGATCTCTGGA-3′ | 5′-GCCGTCTCCCATATCGTTCATGCCC-3′ |
| CtsZ | Cathepsin Z | 5′- GGCGTCGTCGGGGTCGGTGCAGCA- 3′ | 5′- CTGCGCCCCAGCAGAGCCAGCTG- 3′ |