. 2018 Aug 21;9:1950. doi: 10.3389/fmicb.2018.01950

Table 1.

Strains and plasmids used in this study.

Strain or plasmid	Relevant genotype/phenotype	Reference
Strains
PAO1	P. aeruginosa laboratory strain	Stover et al., 2000
PAO1ΔglpT	glpT mutant from PAO1	This work
Ps.a-682	P. aeruginosa clinical isolate from sputum	This work
MG1655	E. coli K12, wild-type, reporter strain	Blattner et al., 1997
MC4100	E. coli K12, lacking the endogenous lacZ gene	Casadaban, 1976
Rosetta^TM(DE3)	T7-expression strain, Cm^R	Novagen/EMD Bioscience

Plasmids
pEX18Gm	Suicide vector with a sacB gene; Gm^R	Hoang et al., 1998
pBBR1MCS4	Broad range host vector; Ap^R	Kovach et al., 1995
pBBR1MCS4lacZ	pBBR1MCS4 with promoterless lacZ; Ap^R	This work
pBBRglpT(PAO1)-P	glpT promoter reporter from PAO1; Ap^R	This work
pBBRglpT(Ps.a-682)-P	glpT promoter reporter fromPs.a-682; Ap^R	This work
pBBRfosA(PAO1)-P	fosA promoter reporter from PAO1; Ap^R	This work
pBBRglpR(PAO1)-P	glpR promoter reporter from PAO1; Ap^R	This work
pNN387	Single copy plasmid with promoterless lacZ; Cm^R	Elledge and Davis, 1989
pNNglpT(PAO1)-P	glpT promoter reporter from PAO1; Cm^R	This work
pBBR1MCS5	Broad range host vector; Gm^R	Kovach et al., 1995
pBBR1MCS5glpT	GlpT expression; Gm^R	This work
pTrc99A	Vector for IPTG-inducible expression; Ap^R	Kurabayashi et al., 2015b
pTrc99Aanr	ANR expression in E. coli; Ap^R	This work
pQE80L	Vector for expression of His-tagged protein; Ap^R	Qiagen
pQE80anr	N-terminal His₆-ANR overexpression plasmid; Ap^R	This work

Ap^R, ampicillin resistance; Cm^R, chloramphenicol resistance; Gm^R, gentamicin resistance.