Table 2.
Primers used in this study.
| Primer | DNA sequence (5′–3′) | Use |
|---|---|---|
| glpT-PA-delta1 | gcgggatccgtcggcgtgttctggggatc | glpT deletion construction |
| glpT-PA-delta2 | gagccgccgcgtcatcagccggctccgaacatcgcgagctccg | glpT deletion construction |
| glpT-PA-delta3 | aagcggagctcgcgatgttcggagccggctgatgacgcggcgg | glpT deletion construction |
| glpT-PA-delta4 | gcgaagcttatctggcggaactgcccgac | glpT deletion construction |
| lacZ-F | gcgggtacctttcacacaggaaacagctatg | pBBR1MCS4lacZ construction |
| lacZ-R | gcctctagattatttttgacaccagaccaac | pBBR1MCS4lacZ construction |
| glpT-PA-PF-NsiI | gcgatgcatcgccctcggccagcgcagg | pBBRglpT(PAO1)-P and pBBRglpT(Ps.a-682) construction |
| glpT-PA-PR-KpnI | gcgggtaccgcgagctccgcttgttgttg | pBBRglpT(PAO1)-P and pBBRglpT(Ps.a-682) construction |
| fosA-PF | gcgatgcatatggtcgaggtcgacgtgc | pBBRfosA(PAO1)-P construction |
| fosA-PR | gcgggtaccgggggctccttgcaagatg | pBBRfosA(PAO1)-P construction |
| glpR-PF | gcgatgcatagccggagtgcgacgagcc | pBBRglpR(PAO1)-P construction |
| glpR-PF | gcgggtaccgggttgttctcgtgctgcc | pBBRglpR(PAO1)-P construction |
| glpT-PA-PF-NotI | gcggcggccgccgccctcggccagcgcagg | pNNglpT(PAO1)-P construction |
| glpT-PA-PR-HindIII | gcgaagcttgcgagctccgcttgttgttg | pNNglpT(PAO1)-P construction |
| pBBRglpT-PA-F | gcgggtaccgcgggccggagcgatacac | pBBR1MCS5glpT construction |
| pBBRglpT-PA-R | gcgaagctttcagccggcttgctgcggc | pBBR1MCS5glpT construction |
| pTrcanr-F | gcgccatggccgaaaccatcaaggtgc | pTrc99Aanr construction |
| pTrcanr-R | gcgggatcctcagccttccagctggccgc | pTrc99Aanr construction |
| pQE80anr-F | gcgggatccgccgaaaccatcaaggtgcg | pQE80anr construction |
| pQE80anr-R | gcgaagctttcagccttccagctggccg | pQE80anr construction |
| glpT-PA-PR-6FAM | tcgcgagctccgcttgttg | DNase I footprinting analyses |
| glpT-PA-RACE1 | cagccgttgatgaagagcagg | 5′-RACE analysis |
| glpT-PA-RACE2 | ggccgaacggcaggaagacc | 5′-RACE analysis |
| glpT-PA-RACE3 | tggcgatggccgacatcgcg | 5′-RACE analysis |