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. 2018 Aug 21;9:899. doi: 10.3389/fphar.2018.00899

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Copyright © 2018 Barbieri, Würth, Pattarozzi, Verduci, Mazzola, Cattaneo, Tonelli, Solari, Bajetto, Daga, Vicentini, Mazzanti and Florio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of human glioblastoma stem cells. Representative images of GSC (derived from GBM6) spheroids (A) and monolayer on Matrigel (B) cultured in stem cell medium supplemented with EGF and bFGF and differentiated cells (C) obtained by switching culture conditions from growth factor-deprived to FBS-containing medium. Left panels reproduce phase contrast images at different magnification (10× and 20×). Right panels depict immunofluorescence for stem cell markers OLIG2, nestin, and Oct4 (A,B), and the glia (GFAP) and neuron (MAP-2) differentiation markers (C). GFAP and MAP-2 expression is depicted from the same microscope field, showing in most cells the co-expression of both markers. Similar results were obtained analyzing all the GSC cultures that enter the study.