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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Melanoma Res. 2018 Oct;28(5):390–397. doi: 10.1097/CMR.0000000000000474

Figure 3. Pre-treatment of the A375 melanoma cell line with MLN4924 sensitizes cells to vemurafenib treatment.

Figure 3

The A375 cell line was treated with 1200 nM MLN4924 for either an initial 24 hours or the entire 72 hours of culture. Vemurafenib treatment (5 μM) was initiated at the 24-hour mark for a total treatment of 48 hours. For some of the treatments, media containing MLN4924 was removed and replaced after the first 24 hours to prevent the mixing of MLN4924 with vemurafenib (R). NR indicates vemurafenib was added directly to MLN4924-contained culture media. Apoptosis was assessed via Annexin V/PI flow cytometry. *p<0.05