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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Exp Eye Res. 2018 Jun 1;174:107–112. doi: 10.1016/j.exer.2018.05.033

Fig. 1.

Fig. 1

WNT7A and WNT7B promote vascular proliferation in vitro. (A) Tissue sections prepared from choroidal neovascular membranes excised from patients with wet AMD were probed with antibodies specific for active non-phosphorylated β-catenin (red) and CD31 (green) by immunohistochemistry. Areas of co-localized β-catenin and CD31 staining are indicated in yellow. White arrows indicate blood vessels. DAPI (blue) was used to counterstain cell nuclei. Scale bars: 25 µm. (B) HMVEC proliferation was stimulated by recombinant human WNT7A and WNT7B (1-way ANOVA with post hoc test for linear trend). Graphs indicate the mean ± SEM of 3 independent experiments. (C–D) WNT7A and WNT7B promote vascular sprouting from mouse choroidal explants (1-way ANOVA with post hoc test for linear trend). Representative images are shown in (C; scale bars: 75 µm). Quantifications shown in (D) represent the mean ± SEM vessel growth measured in 4–14 explants.