Table 2.
List of primary antibodies
| Antibodies | species | specific for | dilution | supplier |
|---|---|---|---|---|
| Bassoon | Mouse | Ribbon synapses | 1:600 | Stressgen (San Diego, CA) |
| Calretinin | Goat | Ganglion and amacrine cells | 1:1000 | Chemicon (Temecula, CA) |
| Ch×10 | sheep | Retinal progenitor cells | 1:200 | Thermo Fisher (Huntington Beach, CA) |
| Cone Transducin | Rabbit | Cone outer segments | 1:200 | Cytosignal (Irvine, CA) |
| CRALBP | Rabbit | Cellular retinaldehyde binding protein; RPE and Müller cells | 1:1000 | Dr. Saari, Univ. of WA (Saari et al., 1984) |
| GFAP | Mouse | Reactive Müller cells | 1:100 | Cell Signaling (Danvers, MA) |
| Iba-1 | Rabbit | Microglia | 1:100 | Biocare Medical (Pacheco, CA) |
| Ku-80 | Rabbit | Human nuclei | 1:400 | Abcam (Eugene, OR) |
| MAP2 | Mouse | Microtubule-associated protein 2 | 1:100 | Millipore/Chemicon (Temecula, CA) |
| MAP2 | Rabbit | Microtubule-associated protein 2 | 1:500 | Chemicon |
| NeuN | Mouse | Neuronal nuclei | 1:400 | Chemicon |
| PKC α | Rabbit | Rod bipolar cells | 1:200 | Oxford Biomedical (Riviera, FL) |
| PKC α | Mouse | Rod bipolar cells | 1:50 | Amersham (Little Chalfont, U.K.) |
| OTX2 | Rabbit | Orthodenticle Homeobox 2 | 1:1000 | Chemicon |
| Recoverin | Rabbit | Photoreceptors, cone bipolar cells | 1:500 | Dr. Dizhoor, Univ. PA (Dizhoor et al., 1991);; Millipore/Chemicon |
| Red Green Opsin | Rabbit | Red and Green cones | 1:2000 | Chemicon |
| Ret. RAX | Rabbit | Retina And Anterior Neural Fold Homeobox Protein | 1:200 | Santa Cruz Biotechnology, Dallas, Texas |
| Rhodopsin (rho1D4) | Mouse | Rods | 1:100 | Dr. Molday, Univ. of British Columbia (Molday and MacKenzie, 1983) |
| Rhodopsin | Rabbit | Rods | 1:2000 | Dr. Plantner, Cleveland, OH (Plantner t al., 1982) |
| Secretagogin | Rabbit | Cone bipolar cells | 1:200 | Cell Signaling |
| α-Synuclein | Rabbit | Rodent amacrine cells and IPL; OLM (general) | 1:100 | Cell Signaling |
| Synaptophysin | mouse | Membrane protein of synaptic vesicles | 1:500 | Sigma |
| Synaptophysin | Goat | Membrane protein of synaptic vesicles | 1:100 | Novus Biologicals (Littleton, CO) |
| SC-121 | Mouse | Cytoplasm of human cells | 1:2K | Stem Cell Inc. (Newark, CA) |
| Vimentin | mouse | Intermediate filament present in most cells | 1:500 | ICN |
| Secondary Antibodies | ||||
| Conjugate | species | specific for | dilution | supplier |
| Alexa Fluor 488 | Donkey | Rabbit IgG (H+L) | 1:400 | Jacson Immuno Research (West Grove, PA) |
| Rhodamine X | Donkey | Rabbit IgG (H+L) | 1:400 | Jacson Immuno Research |
| Rhodamine X | Donkey | Mouse IgG (H+L) | 1:400 | Jacson Immuno Research |
| Alexa Fluor 647 | Donkey | Goat IgG (H+L) | 1:400 | Jacson Immuno Research |