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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Mar 2;142(5):1479–1488.e12. doi: 10.1016/j.jaci.2017.11.059

Figure 5.

Figure 5

Effects of FAM129A on NFκB activation and anti-inflammation effect of glucocorticoid. (A) A549/NFκB-Luc cells were transfected with non-targeting (NT) siRNA or siRNA targeting FAM129A, and 88 h later mRNA levels of FAM129A gene were measured using qPCR and normalized to the level in control siRNA transfected cells. (B) A549/NFκB-Luc cells were transfected with siRNA as in (A). 72 h after transfection, cells were treated with 5 ng/mL IL1β for 16h. The luciferase (Luc) activity was measured and normalized with the amount of total protein. The Luc level was shown relative to that of untreated NT siRNA transfected cells. (C) In the same experiment as shown in (B), additional groups of cells were treated with 5 ng/mL IL1β+100 nM DEX and the Luc level normalized with that upon 5 ng/mL IL1β treatment to determine the percentage values shown. **, P<0.01; ***, P<0.005.