Table 5.
The Detailed Information on Primary Antibodies Used for Western Blots, Immunocytochemistry, and Immunohistochemistry
| Antibody | Vendor | Source | Working Dilution |
|---|---|---|---|
| PAK1 | CST | rabbit | WB: 1:1,000, ICC:1:400 |
| KDR | Abcam | rabbit | WB: 1:1,000 |
| PDK1 | CST | rabbit | WB: 1:1,000 |
| ZNF367 | Abcam | rabbit | WB: 1:500 |
| Phos-ERK1/2 | CST | rabbit | WB: 1:1,000 |
| ERK2 | Santa Cruz Biotechnology | mouse | WB: 1:200 |
| Phos-AKT | Abcam | rabbit | WB: 1:1,000 |
| P85 | Santa Cruz Biotechnology | goat | WB: 1:200 |
| AKT | Abcam | rabbit | WB: 1:1,000 |
| PCNA | Abcam | rabbit | WB: 1:1,000, IHC:200 |
| Cyclin A | Santa Cruz Biotechnology | rabbit | WB: 1:200 |
| Cyclin B1 | Santa Cruz Biotechnology | rabbit | WB: 1:200 |
| Cyclin D1 | Santa Cruz Biotechnology | rabbit | WB: 1:200 |
| Cyclin E | Santa Cruz Biotechnology | mouse | WB: 1:200 |
| CDK2 | Santa Cruz Biotechnology | rabbit | WB: 1:200 |
| GPR125 | Abcam | rabbit | ICC:1:200 |
| GFRA1 | Abcam | goat | ICC:1:200 |
| CD90 | Abcam | rabbit | ICC:1:200 |
| UCHL1 | AbDSerotec | mouse | ICC and IHC:1:200 |
| RET | Santa Cruz Biotechnology | rabbit | WB: 1:200 |
| DAZ2 | Abcam | rabbit | WB: 1:1,000 |
| DAZL | Abcam | rabbit | IF:1:100 |
| VASA | Santa Cruz Biotechnology | goat | IF:1:100 |
| WT1 | Santa Cruz Biotechnology | rabbit | IF:1:100 |
| GATA4 | Santa Cruz Biotechnology | goat | IF:1:100 |
| Ki67 | BD Biosciences | mouse | IHC:1:200 |
| PLZF | Santa Cruz Biotechnology | rabbit | IHC:1:200 |
| SV40 | Santa Cruz Biotechnology | mouse | WB: 1:200 |
| ACTB | Proteintech | mouse | WB: 1:5,000 |
WB, Western blots; ICC, immunocytochemistry; IHC, immunohistochemistry.