Fig. 1.

Hsp70 and IgG antibodies were linked to Dynabeads, and T24 bladder cancer cell lysates were immunoprecipitated. SDS-PAGE, followed by silver staining, was performed and the band at the arrow was sliced from the gel and analyzed by LC-MS/MS. The unknown band was determined to be keratin 9. a Hsp70-Dynabead-linked Immunoprecipitation of T24 lysates in the absence of VER155008 (VER-) and presence of VER155008 (VER+). Lane C is an IgG-Dynabead-linked immunoprecipitate of a DMSO-treated T24 lysate (IgG control). Molecular weight markers (MWM) of 25, 50, and 75 represent molecular weights in kilodaltons. b bold black and red letters represent keratin 9 amino acid fragments identified from the excised band by LC-MS/MS. Red letters identify the amino acids found in the filament super family