Fig. 4.

NvHsp70 isoforms provide essential Hsp70 function in yeast. Ssa1–4∆ cells were transformed with control plasmid pAG415-ccDB, Ssa1-expressing, or NvHSP70 isoform-expressing plasmids and then serially diluted onto media lacking leucine or containing 5-FOA. Growth of cells on 5-FOA demonstrates ability of NvHSP70 isoforms to provide essential function when expressed as the sole Hsp70 in the cell. Plates were incubated for 3 days at 30 °C and then photographed