Fig. 1.

Validation of the level of luciferase activity derived from the hspa1b/luciferase transgene as quantitative reporter of heat-induced heat-shock response. a Bovine cells were exposed to heat shock for different incubation times, and luciferase activity was recorded at various times post-treatment. b Luciferase activity and cell viability were recorded 5 h after various heat-shock treatments. The results shown are the mean ± SD of at least three independent experiments. c Bovine cells were exposed to 45 °C for the indicated periods of time and total soluble protein extracts prepared 5 h post-treatment. Induction of luciferase and HSP70/HSPA were analyzed by western blotting. Actin detection was used as loading control