Fig. 5.

Evaluation of redox changes following the four types of oxidative stress and heat shock. Bovine primary cells were grown in the presence (+) or absence (−) of 0.5 mM BSO and treated as described in Fig. 3. Immediately post-treatment, cells were either loaded with the ROS probes CM-H₂DCFDA and DHR123 (N = 3 for all, except N = 2 for CM-H₂DCFDA after DA) and analyzed by flow cytometry (a) or lysed in lysis buffer without β-mercaptoethanol and the profiles of protein glutathionylation (N = 3) determined by western blot analysis (b). Shown is a representative image of the western blot analyses