Table 1.
Primers used for qPCR analysis
| Primer | Forward | Reverse |
|---|---|---|
| GAPDH | TGTGTCCGTCGTGGATCTGA | TTGCTGTTGAAGTCGCAGGAG |
| HPRT | AGTACAGCCCCAAAATGGTTAAG | CACAAACGTGATTCAAATCCCTG |
| TBP | AAGAGAGCCACGGACAACTGC | CTTCACATCACAGCTCCCCAC |
| PGC-1α | GCTCATTGTTGTACTGGTTGGATATG | CGTAGGCCCAGGTACGACAG |
| Myoglobin | CCCTGGAGGGTTGAGCACGGT | AGGCCACCTGGTCCTGAAGGG |
| SDHD | TCATGGGGACACCCTGCCGA | TCCACAGCATGGCAACCGCTC |
| CS | GCCCAGCAGCTGTAGGATGACC | GGGGCCTGTCCCTGGCGTA |
| MyH7 (Myosin Ib) | CCTTGGCACCAATGTCCCGGCTC | GAAGCGCAATGCAGAGTCGGTG |
| MyH2 (Myosin IIa) | ATGAGCTCCGACGCCGAG | TCTGTTAGCATGAACTGGTAGGCG |
| MyH1 (Myosin IIx) | GCTTCAAGTTTGGACCCACG | ACTTCCGGAGGTAAGGAGCA |
| MyH4 (Myosin IIb) | GTGATTTCTCCTGTCACCTCTC | GGAGGACCGCAAGAACGTGCTGA |
| Actg1 | TTCCTTCCTGGGCATGGAGT | GGCATACAGGTCTTTGCGGA |
| Murf1 | GCAGCTCATCAAGAGCATTGT | CCAAAGTCAATGGCCCTCAA |
| Atrogin-1 | GTGAGGACCGGCTACTGTGG | CAATCCAGCTGCCCTTTGTC |
| Traf6 | GCGAGAGATTCTTTCCCTGACG | TTGGCACTGGGGACAATTCAC |
| Calpain 1 | CTGGAGGCTGCAGGAACTAC | CTCCCGGTTGTCATAGTCGT |
| Calpain 3 | CGAGAAACGAGGGGAAACCC | ACAGATCTCCATGTCGTCGC |
| Calpastatin | TGGTGGTACAAGACGCATGTTA | ACATGTGGTCTGGAAGGCTG |
| MARCKS | CCAGTTCTCCAAGACCGCAG | CATTCTCCTGCCCATTTGCTTTG |
| Hsp70 | AGTCCGACATGAAGCACTGG | ATCTCCTCCGGGAAGAA |
| Hsp90aa | TGTTCATTCAGCCACGATGC | AACTGGGCAATTTCTGCCTG |
| Hsp90ab | CAGACCATGGTGAGCCCATT | CACCACTTCCTTGACCCTCC |
| Stub1 | CCCTGATAAGAGCCCGAGTG | ACAAGTGGGTTCCGAGTGATG |
| UFD2 | GTCACCAGACCGCAATCTCA | GGCTCCCAAACTAGACAACGA |
| CryaB | ACGTGAAGCACTTCTCTCCG | ATGTTCGTCCTGGCGTTCTT |
| TCP1 | TTAAACGGTCTCAGAGCGGA | GCAGCCATAACATTCTGGGA |
| FKBP8 | GCTGGGAGACTGCGATGTTA | TGTATGGGCTCCTGCCCT |
| skNAC | ACAGCCTCAGGCTGAGACAG | TTTGAAGTTCCTTTTGCCCAG |
| Smyd1 | GCATCTTCCCCAACCTGGGCCT | GGGCCCGGAGCTCAATCCTCAT |
List of all primers employed for semi-quantitative RT-PCR analysis in this study. Commercially designed primers (Qiagen QuantiTect Primer Assays): Titin, Nedd4, Mical, MyoD1, and Cmya4