Figure 1.

(a) TCS haplotype network (D-loop, 830 bp) for Astatotilapia of Lake Chala (dark blue), Astatotilapia cf. bloyeti of the lower Pangani (red) and of Nyumba ya Mungu (black), Astatotilapia sparsidens of Lake Manyara (light blue), Astatotilapia sp. of Lake Babati (green) and Astatotilapia sp. of the Ruaha Swamps (olive). Numbers at the pie charts state number of individuals per sampling location. Asterisks indicate individuals that were included in the phylogenetic tree. (b) Map indicating sampling sites. (c) Phylogenetic tree based on approximately 2 million sites indicating monophyly of Astatotilapia sp. ‘Chala’ with its closest relative being A. cf. bloyeti from the close-by Pangani River catchment (red and black). Colour code as in (a,b), with representatives of the Lake Victoria Region Super-flock in yellow (Pundamilia spp. and Enterochromis spp.), and Metriaclima zebra of Lake Malawi in grey. Coloured tip extensions within the Lake Chala clade represent the morph assignments (blue, BBE; black, GAL; green, LEA; light green, LEO; brown, OSH; purple, PLB; violet, PLR; yellow, YBE). Bootstrap supports greater than 70% are given. (Online version in colour.)