Figure 2.
Size exclusion venom profiles for Spilotes sulphureus and Boiga irregularis, with SDS-PAGE results of S. sulphureus peaks. Crude venoms (3 mg) from S. sulphureus (a) and B. irregularis (b) were fractionated using size exclusion HPLC; protein identities are based on SDS-PAGE results, N-terminal sequencing, LC-MS/MS, and enzymatic assays. Reduced and non-reduced SDS-PAGE gels are shown for the S. sulphureus chromatogram (c); crude venom (V) was also run under non-reduced conditions. HPLC chromatograms for reduced sulditoxin (d, arrows) and reduced sulmotoxin 1 (e, arrow) show the presence of two and one peaks, respectively; other peaks (approx. 5.4 & 12 min, unlabelled) are reduction chemistry peaks. Abbreviations: Mr = relative molecular masses, 3FTx = three-finger toxin, CRiSP = cysteine-rich secretory protein, and SVMP = snake venom metalloproteinase. (Online version in colour.)