Figure 4.

Comparison of extract of (A) UV-reflectant petal parts and (B) UV-absorbent petal parts (H. calycinum) by HPLC, with detection at 350 nm, showing the order of elution of pigments 1–7 (MAU, milliabsorbance units). Both extracts were injected as standard solutions consisting of 4.0 mg of crude extract per milliliter of methanol. The elution was carried out by using acetonitrile containing 0.5% formic acid. Pigments 1 and 2, which coeluted under these conditions (the peak at 1.6 min), were subsequently resolved in a separate HPLC step (Insets).