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. 2018 Jul 17;40(4):1875–1884. doi: 10.3892/or.2018.6579

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Silencing of CDCA5 inhibits HCC cell proliferation and clonogenicity. (A and B) Quantitation of CDCA5 mRNA and protein levels in MHCC97-H cells by qRT-PCR and western blotting after transfection with different siRNAs targeting CDCA5 (si1, si2 and si3) or a negative control siRNA (NC). (C) CDCA5 expression in MHCC97-H and Huh7 cells after transfection with si3 and NC. (D) The proliferation curves of MHCC97-H and Huh7 cells after CDCA5 knockdown. (E) A colony formation assay was used to measure the clonogenicity of MHCC97-H and Huh7 cells after CDCA5 knockdown. *P<0.05. CDCA5, cell division cycle associated 5; HCC, hepatocellular carcinoma.