Table 3.
Overall results from the intra-run repeatability and inter-run reproducibility tests on the detection of two RNA targets, using Protocol 3. The second and third columns display the mean and standard deviation (Std. Dev.) of Cq values from each of the three runs executed in three Q3-Plus V2 (Q3) instruments and in one CFX96 instrument, for both targets. The fourth column (Mean Std. Dev.) shows the average of the three intra-run standard deviation values for both targets in each platform—Q3 and CFX96. The fifth and sixth columns show the inter-run—that is, the overall—mean and standard deviation of Cq values for both targets in each platform.
| Intra-Run | Inter-Run | ||||
|---|---|---|---|---|---|
| Mean | Std. Dev. | Mean Std. Dev. | Mean | Std. Dev. | |
| Q3, Target 1 |
26.0 | 0.058 | 0.063 | 26.0 | 0.076 |
| 26.0 | 0.075 | ||||
| 26.1 | 0.058 | ||||
| CFX96, Target 1 |
26.6 | 0.273 | 0.158 | 26.5 | 0.207 |
| 26.5 | 0.126 | ||||
| 26.4 | 0.075 | ||||
| Q3, Target 2 |
19.3 | 0.050 | 0.069 | 19.2 | 0.073 |
| 19.2 | 0.090 | ||||
| 19.2 | 0.069 | ||||
| CFX96, Target 2 |
19.4 | 0.223 | 0.192 | 19.3 | 0.217 |
| 19.3 | 0.149 | ||||
| 19.1 | 0.205 | ||||