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. 2018 Jul 24;40(4):1843–1854. doi: 10.3892/or.2018.6593

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Let-7a inhibits cell cycle progression. (A) Representative cell cycle histograms show the percentage of A549 cells in each phase of the cell cycle after miR-negative control (scrambled), let-7a-mimics or let-7a-inhibitor transfection. (B) Let-7a resulted in cell cycle arrest at the G₀/G₁ in H1299 cells, compared to the control. (C) The expression of Rb and p-Rb proteins were analyzed by western blotting in A549 cells. (D) The expression of Rb and p-Rb proteins in H1299 cells. GAPDH was used as an internal control.