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Primer sequences and molecular size of the product
| Gene | Primer sequences 5’- 3’ | Product |
|---|---|---|
| Col-I | F: ATGGTGGCAGCCAGTTTG | 328 bp |
| R: AGGAATGGCAGGCGAGAT | ||
| Col-III | F: AAGAGCGGAGAATACTGGG | 532 bp |
| R: CAATGTCATAGGGTGCGATA | ||
| R: CTCACCAGTCGGGTCTCAGTA | ||
| β-actin | F: GAGGGAAATCGTGCGTGAC | 445 bp |
| R: CTGGAAGGTGGACAGTGAG |
F: Forward; R: Reverse; Col-I: Type I collagen; Col-III: Type III collagen.
