Figure 1.

Immunohistochemical analysis for Sirtuin 1 (SIRT1). Representative images of SIRT1 detection in subcutaneous abdominal fat from obese patients with pre-diabetic condition and obese patients with normo-glycemic condition. Immunoistochemistry of obese patients with pre-diabetic condition in metformin therapy (group 1, left upper part, A) vs. obese patients with pre-diabetic condition in placebo therapy (group 2, right upper part, B) vs. obese patients with normo glycemic condition (group 3, in the left inferior part, C); In the right inferior part (D), bar graph expression and matched western blot expression of SIRT1 in obese patients with pre-diabetic condition in metformin therapy (group 1, red color), obese patient with pre-diabetic condition in placebo therapy (group 2, gray color), and obese patients with normo glycemic condition(group 3, green color). Protein levels determined by Western blot analysis of adipose abdominal tissue homogenates from obese patients with normo-glycemic condition and obese patients with pre-diabetic condition. Inset, representative image of Western blot analysis (D). Lanes 1, obese patient with pre-diabetic condition in metformin therapy (group 1). Lanes 2, obese patient with pre-diabetic condition in placebo therapy (group 2). Lanes 3, obese patient with normo-glycemic condition (group 3). Results are mean ± SD of 5 independent experiments. Group 1 vs. group 3, ^*P <0.05; group 2 vs. group 3, ^**p <0.05.