Figure 2.

Immunohistochemical analysis for Fas. Representative images of Fas detection in subcutaneous abdominal fat from obese patient with pre-diabetic condition and obese patient with normo-glycemic condition. Immunoistochemistry of obese patient with pre-diabetic condition in metformin therapy (group 1, left upper part, A) vs. obese patient with pre-diabetic condition in placebo therapy (group 2, right upper part, B) vs. obese patient with normo glycemic condition (group 3, in the left inferior part, C); In the right inferior part (D), expression of FAS in obese patient with pre-diabetic condition in metformin therapy (group 1, red color), obese patient with pre-diabetic condition in placebo therapy (group 2, gray color), and in obese patient with normo glycemic condition (group 3, green color). Results are mean ± SD of 5 independent experiments. Results are mean ± SD of 5 independent experiments. Group 1 vs. group 3, ^*P <0.05; group 2 vs. group 3, ^**p <0.05.