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. 2018 Jun 4;165(1):31–39. doi: 10.1093/toxsci/kfy174

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Published by Oxford University Press on behalf of the Society of Toxicology 2018. This work is written by US Government employees and is in the public domain in the US.

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Figure 1. — The mESCs test. A, Viability of pluripotent murine D3 ESCs and differentiated 3T3 fibroblasts is compared across an 8-point dose-response following 10 days of toxicant exposure. B, EBs are generated via the hanging drop method, and allowed to differentiate into contracting cardiomyocytes while being exposed for 10 days across an 8-point dose-response. C, EB formation via the hanging drop method. Compound concentrations that reduce cell viability (IC₅₀) and cardiomyocyte differentiation (ID₅₀) by 50% are calculated and input into the EST prediction model to determine the embryotoxic potential of the test compound. D, Hypothetical example of concentration-response curves for an embryotoxic compound that inhibits cardiomyocyte differentiation at non-cytotoxic concentrations. E, Hypothetical example of concentration response curves for a nonembryotoxic compound that only inhibits cardiomyocyte differentiation at cytotoxic concentrations.