Figure 7.

Gps suppressed NLRP3 inflammasome activation by inhibiting ROS release. A, The levels of NLRP3 inflammasome markers were assayed via immunoblotting in the medium supernatants (Sup) and lysates (Lys) of H9C2 cells treated with Gps for 48 h. B‐C, The levels of IL‐1β (B) and IL‐18 (C) proteins in medium supernatants of H9C2 cells were analysed by ELISA after being treated with Gps for 48 h. D‐E, ROS release was detected by FCM in H9C2 cells treated with Gps for 48 h (D) and the statistical result of positive cells (E). F, The cytoplasm and mitochondrion of H9C2 cells were analysed by immunoblotting for cytochrome c after treating with Gps for 48 h. Data are shown as mean ± SD. **P ≤ .01, ***P ≤ .001