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NEDD4‐mediated ubiquitination of PIP5Kα. FLAG‐PIP5Kα was cotransfected into HEK293 cells together with HA‐ubiquitin and/or V5‐NEDD4 (A) or with HA‐NEDD4 and WT or K48R HA‐ubiquitin (Ub) (C). (B) HEK293 cells were treated with control siRNA or NEDD4 siRNAs and were then transfected with FLAG‐PIP5Kα. (A‐C) Cell lysates (input) were subjected to FLAG IP, and the input and FLAG IP samples were analysed by Western blotting with the indicated antibodies
