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. 2018 Jun 17;22(9):4209–4220. doi: 10.1111/jcmm.13701

Figure 2.

Figure 2

Curcumin inhibits the acidic bile‐induced NF‐κB activation and bcl‐2 overexpression in human hypopharyngeal primary cells (HHPC). Western blot analysis is performed in nuclear and cytoplasmic protein extracts of HHPC treated with and without curcumin, for p‐NF‐κB (p65 Ser536), p‐IκB‐α (Ser32/36) and bcl‐2. A, Normalized protein levels of (a) nuclear p‐NF‐κB(p65 Ser536), (b) cytoplasmic p‐IκB‐α (Ser32/36) and (c) cytoplasmic bcl‐2 (by t test; multiple comparisons by Holm‐Sidak; GraphPad Prism 6.0). B, Normalized protein ratios (with/without curcumin) of (a) nuclear p‐NF‐κB(p65 Ser536), (b) cytoplasmic p‐IκB‐α (Ser32/36) and (c) cytoplasmic bcl‐2, between different experimental and control HHPC‐treated groups (one‐way ANOVA; Kruskal‐Wallis, *P < .05; **P < .005; GraphPad Prism 6.0) (Nuclear p‐NF‐κB protein levels are normalized to histone 1; cytoplasmic p‐IκB‐α and bcl‐2 protein levels are normalized to β‐actin. Data are derived from three independent assays)