Figure 4.

Glomerular fibrin deposition and kidney p38 and c‐Jun amino terminal kinase activation in day 14 nephrotoxic serum nephritis (NTN). Disease was induced in groups of 8 WKY rats which were treated with GS‐444217 (drug; NTN‐D) or vehicle (NTN‐V) alone. A‐C, Immunostaining for fibrinogen: A, little staining is seen in normal rat kidney; B, pronounced fibrin deposition is seen in the glomerular tuft and in crescents in vehicle‐treated disease, (C) drug treatment partially reduces fibrin deposition in day 14 NTN. D, Quantification of the glomerular fibrin staining score (0‐3+). E, Western blot analysis of whole kidney lysates for p‐p38 and p‐c‐Jun with tubulin reprobe. Graphs show quantification for p‐p38 (F) and p‐c‐Jun (G). Data are mean ± SD. D, Analysis by nonparametric one‐way ANOVA with Dunn's multiple comparison test; F,G, analysis by one‐way ANOVA with Tukey's multiple comparison test