FIGURE 6.

Butyrate extensively ameliorated the changes in fecal metabolomic profile induced by the MCD diet. (A) A 4-state model of PCA plot comparing between the Control (red), MCD (blue), Control + SoB (orange), and MCD + SoB (purple) groups. Q² (cum) = 0.222 and R²X (cum) = 0.550. (B) OPLS-DA score scatter plot comparing between the Control (red), MCD (blue), Control + SoB (orange), and MCD + SoB (purple) groups. Q² (cum) = 0.909 and R²Y (cum) = 0.963. (C) OPLS-DA score scatter plot comparing the MCD (blue) and MCD + SoB (purple) groups. Q² (cum) = 0.950 and R²Y (cum) = 0.990. Each symbol represents one sample (N = 24 per group, with a quadruplicate technical replicates of six samples per group, except for the MCD + SoB group, for which three unqualified data set datasets were eliminated). (D) Euclidean distance hierarchical clustering analysis demonstrating the different intensity levels of characteristic metabolites.