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. 2018 Aug 1;18(8):2461. doi: 10.3390/s18082461

Figure 5.

Figure 5

Simple spectral deconvulsion approaches to scattering labels. Illustrated is the predicted scattering cross-section ratio of 60 nm Ag to 60 nm Au particles (A) and acquired ratio of Ag to Au (B). Vertical red lines represent the particle scattering wavelengths (405, 488, 561, 640 nm) collected on the Astrios EQ instrument. The acquired scattering channel data for CFSE-stained EVs, 60 nm Ag and 60 nm Au particles on 488 nm and 561 nm SSC channels (C). The green area indicates a higher scattering power at 561 nm, while the red area indicates a higher scattering power at 488 nm. The red dotted line indicates an equal scattering power at 561 nm and 488 nm.