Figure 3.

Effect of Myricitrin on RANKL‐induced the mRNA expression of c‐Fos and NFATc1. A, Western blotting analysis showed myricitrin inhibits the expression of c‐Fos and NFATc1 induced by RANKL. The intensities of the protein bands were analysed and normalized to actin. Similar results were obtained in at least 3 independent experiments. B and C Bone marrow macrophage cells were pretreated with or without myricitrin (5 μmol/L) for 1 h and with RANKL (100 ng/mL) for the indicated time (*P < .05 vs. Sham; ^# P < .05 vs. DMSO). The mRNA expression of c‐Fos and NFATc1 genes was analysed by real‐time RT‐PCR