Figure 2.

Generation of LINC00310 KO in LM‐4142 cells with CRISPR/Cas9 system using a dual gRNA approach. A, LINC00310 was up‐regulated in breast cancer cell line LM‐4142 compared with the non‐malignant breast cells (HMLE). B, Detection of LINC00310 deletion by genomic PCR using primers LINC00310‐outside‐5.1 and LINC00310‐outside‐3.1. The red arrow indicates deletion bands. C, Detection of LINC00310 target region using genomic DNA as a template and primers LINC00310‐RT‐5.1 and LINC00310‐RT‐3.1. The red arrow indicates inside bands of the targeting sites. D, Expression of LINC00310 in KO cells compared with the vector control by qRT‐PCR. **P < .01