Figure 2.

Deoxycholic acid stimulated the release of amphiregulin (AREG), but not transforming growth factor (TGF)‐α or heparin‐binding (HB)‐EGF in intestinal tumour cells. A‐C,E, The concentration of epidermal growth factor receptor ligand TGF‐α, HB‐EGF and AREG by ELISA assay in Immorto‐Min colonic epithelial cell line (IMCE) and human colorectal cancer cell line (HCT‐116) cell lines treated with deoxycholic acid (DCA) along with time variation. D,F, Real‐time PCR results showed that AREG mRNA levels peaked at 2 h of incubation with DCA in HCT‐116 cells, whereas no change significantly occurred after DCA exposure in IMCE cells. DCA, deoxycholic acid. *, P < .05, **, P < .01, ***, P < .001. Data were quantified from at least 3 separate experiments