Figure 1.

Identification of Dnmt3a KO using the CRISPR/Cas9 system in CHO‐K1 cells. A, PCR amplification for Dnmt3a gene in the monoclones of CHO‐K1 cells.. Six monoclones (3a‐30, 31, 32, 33, 40 and 41) harbouring indel mutations, which cause PCR product length polymorphisms, were selected as Dnmt3a‐deficient mutants. B, Sequencing analysis of Dnmt3a KO in the monoclones 3a‐30 and 40. Sequencing results show that frame shift mutation (red arrow) occurred in the target region of the Dnmt3a gene (the bases in red). Sequencing primers are underlined. sgRNAs for Dnmt3a KO are denoted by with wavy lines