Table 1.
Primers used for PCR amplification and gene knockout
| Gene | Primers | Sequences (5′‐3′) | Size, bp |
|---|---|---|---|
| Dnmt3a 3 + 4 | D3a‐Ex1seq‐L | GACCACAAGAATTCCGGCTC | 493 |
| D3a‐Ex1seq‐R | CGTGTGTGAATCTGTGTGGG | ||
| sgRNA1 | D3a‐Ex1‐98rev | ATCATCCTCCCGCTCCAAAGTGG | |
| sgRNA2 | D3a‐Ex1‐308fw | TTTGAGGGGTCATCCTTGCAGGG | |
| CMV promoter | CMV_8F_10F | aggaagagagTGATTTTATGGGATTTTTTTATTTGG | 284 |
| CMV_8F_T7R | cagtaatacgactcactatagggagaaggctTTCTCTAATTAACCAAAAAACTCTACTT | ||
| EF‐1α promoter | EF‐1α_2F_10F | aggaagagagTTATTATTGAGGTGGAGAAGAGTATG | 459 |
| EF‐1α_2F_T7R | cagtaatacgactcactatagggagaaggctCAAACCAAACCTCAACTCAAACA | ||
| Dnmt3a for qPCR | F | CGATGAACCGGAGTACGAGG | 179 |
| R | CCACTGAGAACTTGCCGTCT | ||
| GAPDH for qPCR | F | CCGCATCCCTGAGACAAGAT | 175 |
| R | TGCCGTGGGTGGAATCATAC |