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Fn14 knockdown ameliorated HL‐1 atrial myocytes hypertrophy induced by TWEAK stimulation. A, B Western blot analysis of Fn14, ANP, Troponin T protein expression in HL‐1 atrial myocytes with Fn14 siRNA knockdown. C, Immunofluorescence for Fn14 (green) and DAPI (blue) demonstrating increased Fn14 levels after TWEAK (100 ng/mL) treatment for 24 h; Fn14 siRNA inhibition attenuated the effect in HL‐1 atrial myocytes. *P < .05 vs control without treatment and # P < .05 vs only TWEAK
