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. 2018 Jul 4;22(9):4317–4327. doi: 10.1111/jcmm.13721

Figure 2.

Figure 2

Dasatinib inhibits platelet P‐selectin expression in both human and mouse platelets and prevents the adhesion of platelets and monocytes to TGF‐β1 stimulated endothelium under flow conditions. A, Representative flow cytometry plots of P‐selectin expression in human or (B) murine platelet‐rich plasma (PRP) prepared from freshly drawn blood and stimulated with 30 μmol/L ADP ± 20 μmol/L Dasatinib. C, Mean fluorescent intensity (MFI) of P‐selectin staining on human or (D) murine platelets. E, The effect of Dasatanib on platelet and (F) monocyte recruitment to TGF‐β1 stimulated endothelial cells. Dasatanib (4 μmol/L) was added to blood 15 min prior to perfusion across EC and the level of monocyte and platelet adhesion assessed by phase contrast microscopy (n = 4). Data are mean ± SD. *P < .05, **P < .01, vs ADP only control (C, D) vs blood perfused in the absence of Dasatanib (E, F) using one‐way ANOVA followed by a Dunnett's post‐test