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. 2018 Jul 4;22(9):4317–4327. doi: 10.1111/jcmm.13721

Figure 3.

Figure 3

Mice deficient in Fgr or Lyn demonstrate decreased platelet activation, P selectin expression and leucocyte recruitment to VWF under flow conditions. A, Representative images of WT (B) Lyn−/− or (C) Fgr−/− platelets activated with 30 μmol/L ADP on a matrix of immobilized VWF in the presence of cells; (D) Average platelet coverage from flow (1000−s) on a matrix of immobilized VWF in the presence of 30 μmol/L ADP. The level of platelet adhesion was assessed by fluorescent microscopy. Data are mean ± SEM. compared to WT (C57Bl6) ADP positive controls (n = 15). E, Representative flow cytometry plot and (F) MFI ± SD of P‐selectin expression on WT, Lyn−/− or Fgr−/− platelets in response to ADP (n = 3). G, Number of WT monocytes adhering to Lyn−/− or (H) Fgr−/− platelets under flow conditions (100−s). The level of monocyte was adhesion assessed by phase contrast microscopy (n = 18). Data are mean ± SEM. Data are shown as mean ± SD. *P < .05, **P < .01 compared to the WT ADP positive control using one‐way ANOVA followed by a Dunnett's post‐test