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The Rcs stress response is induced when uppS31 is present. (A) Induction of the Rcs response was monitored by measuring sfGFP from the rprA reporter plasmid pDKR1. Cells were grown at 42°C in LB to an OD600 of 0.3 to 0.5 and photographed by phase-contrast and fluorescence microscopy. The scale bar represents 3 μm. (B) Cells were grown at 30°C or 42°C in LB. Mean fluorescence intensity is reported in arbitrary units. Strains were CS109 uppS+ (SKCS156/pDKR1) and CS109 uppS31 (CS109/pDKR1).
