TABLE 3.
Wild type or mutant strain | WT or mutationa |
Growth atb: |
Shape atc: |
||||
---|---|---|---|---|---|---|---|
UppS | IspH | Idi | 30°C | 42°C | 30°C | 42°C | |
MG1655 background | |||||||
MG1655 | WT | WT | WT | + | + | + | + |
SKMG173 | 31 | WT | WT | − | ND | − | ND |
SKMG198 | 31 | 269 | WT | + | + | + | − |
WJM11 | 31 | WT | Δ | + | − | − | − |
WJM32 | 31 | WT | IS | + | − | − | − |
SKMG143 | WT | 269 | WT | + | + | + | + |
WJM25 | WT | 269 | Δ | + | + | + | + |
WJM30 | WT | 269 | IS | + | + | + | + |
SKMG124 | WT | WT | Δ | + | + | + | + |
SKMG126 | WT | WT | IS | + | + | + | + |
WJM12 | 31 | 269 | Δ | + | + | + | − |
WJM33 | 31 | 269 | IS | + | + | + | − |
CS109 background | |||||||
WJM27 | WT | WT | WT | + | + | + | + |
WJM31 | 31 | WT | WT | + | + | + | − |
WJM34 | 31 | 269 | WT | + | + | + | − |
WJM26 | 31 | WT | Δ | + | + | + | − |
SKCS183 | 31 | WT | IS | + | + | + | − |
WJM16 | WT | 269 | WT | + | + | + | + |
WJM17 | WT | 269 | Δ | + | + | + | + |
SKCS156 | WT | 269 | IS | + | + | + | + |
WJM29 | WT | WT | Δ | + | + | + | + |
SKCS195 | WT | WT | IS | + | + | + | + |
WJM8 | 31 | 269 | Δ | + | + | + | − |
CS109 | 31 | 269 | IS | + | + | + | − |
WT, wild type; 31, UppSW31R; 269, IspHS269A; IS, IS-idi; Δ, Δidi.
+, cells grew; −, cells did not grow; ND, not determined.
+, wild-type cell shape; −, aberrant cell shape.