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Size of E. coli MG1655 uppS31 ispH269 with or without excess IPP
| Plasmid | Mevalonate (2.5 mM) | No. of cells at 42°C | Cell size (mean ± SD) (μm) |
|
|---|---|---|---|---|
| Length | Width | |||
| Vector | − | 218 | 4.15 ± 1.22 | 1.89 ± 0.25 |
| + | 204 | 4.23 ± 1.27 | 2.01 ± 0.29 | |
| pMevBa | − | 195 | 4.09 ± 1.13 | 1.86 ± 0.28 |
| + | 258 | 3.15 ± 0.89 | 1.41 ± 0.19 | |
Plasmid pMEVB was transformed into MG1655 uppS31 ispH269 to express the enzymes that synthesize IPP from mevalonate.
