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. 2018 Aug 28;17:131. doi: 10.1186/s12934-018-0978-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Biochemical characterization of TubE as a CDG-PRPP transferase. a Schematic of the TubE-catalyzed reaction in the presence of PRPP and CDG. b HPLC analysis of the TubE reaction. TubE reaction, HPLC profile of the TubE reaction using PRPP and CDG as substrates; Negative control, HPLC profile of the reaction using PRPP and CDG as substrates but without TubE added. Compound X shows [M+H]⁺ ion at m/z 327.0923, with main fragment ions at 177.9827, 194.9009, 292.0454, and 309.9970, which are fully consistent with the theoretical fragmentation pattern of dephosphorylated 1